Immunohistochemistry (IHC) in Cancer Research

Jun 24, 2021 11:00:00 AM / by Champions Oncology posted in IHC

Plasmacytoid carcinoma of the urinary bladder – H&E Staining


Immunohistochemistry (IHC) is a technique that originates in the early twentieth century but continues to be a valuable method that forms the backbone of molecular pathology. IHC is used for histological examination of tissues and specifically detects the presence of a molecule, such as a tumor antigen. IHC uses antibody-based labeling in which the primary antibody detects the target of interest and the secondary antibody detects the primary antibody which is linked to a molecule for microscopic visualization. Many different secondary antibody labeling modalities exist, including fluorescence, enzyme-mediated reactions and colloidal gold, and different labels are suited to specific microscopy platforms.

Consider these five aspects of IHC as you implement this technique in preclinical cancer research:

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Applications of IHC for Oncology Research

Apr 15, 2021 11:00:00 AM / by Champions Oncology posted in IHC

Immunohistochemistry (IHC) is one of the oldest and widely used diagnostic techniques that continues to be a valuable tool for immuno-oncology research. IHC is a method for visualizing the tissue localization of specific antigens and has evolved since its development in the 1940s[1]. This method has been an essential diagnostic tool for oncology because it enables pathologists to determine the histological grade of a tumor, which is a critical parameter for predicting the prognosis of tumors and determining the best treatment options. IHC is dependent on the skilled preparation and staining of thin tissue sections that are read by diagnostic pathologists or research scientists.

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Quantitative IHC: At the Cutting Edge of Molecular Pathology for Cancer Diagnostics

Jul 23, 2020 10:11:48 AM / by Champions Oncology posted in IHC

Molecular pathology has been used for decades together with anatomic pathology for disease diagnosis and evaluating treatment efficacy. Immunohistochemistry (IHC) has been a key technique used on molecular pathology that involves staining tissue sections with molecular markers and evaluating staining patterns that may correspond to different tumor stages or other disease pathologies.

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