Brandon Walling, PhD, from Champions Oncology, answers key questions from his recent webinar.
1) I am hearing more about spectral flow cytometry, what is this, and what benefits does it provide?
Spectral flow cytometry differs from conventional flow cytometry through utilizing all detectors to capture the entire spectra for each fluorophore. This provides a “fingerprint” for each fluorophore which can allow for extremely similar emission spectra to be separated (ex. Alexa647 and APC). This can increase the resolution of the sensitivity of your study. Spectral flow cytometry also has cell autofluorescence detection and subtraction capability, which can improve dim marker’s separation from high auto-FL background.